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KMID : 1094720110160061173
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 6 p.1173 ~ p.1179
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Generation of catalytic protein particles in Escherichia coli cells using the cellulose-binding domain from Cellulomonas fimi as a fusion partner
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Choi Su-Lim
Lee Sang-Jun
Ha Jae-Seok
Song Jae-Jun
Rhee Young-Ha
Lee Seung-Goo
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Abstract
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The cellulose-binding domain (CBD) of a Cellulomonas fimi exo-glucanase was translationally fused with β-glucuronidase (GusA) from Escherichia coli and β-glycosidase (BglA) from Thermus caldophilus, respectively. Two fusion proteins (GusA-CBD and BglA-CBD) were expressed as insoluble aggregates in cells and isolated by centrifugation of the cell lysates. Interestingly, activity assays revealed that > 90% of the catalytic activity of both proteins was localized in the insoluble fractions. For example, the GusA-CBD particles exhibited 21 units per mg protein, which corresponded to 19% specific activity of the highly purified soluble GusA. The specific activity increased further up to 42 units per mg protein when treated with either sonication or chaotropic L-arginine. These results demonstrate that fusion with CBD family II may activate catalytic protein particles in E. coli cells, and that internal proteins of the particles are also active. Finally, the protein particles were tested in repeated batch operations after being cross-linked with chemicals, indicating that they have potential as a new preparation for immobilized biocatalysts.
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KEYWORD
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cellulose-binding domain (CBD), protein particles, immobilization, Escherichia coli, β-glycosidase, β-glucuronidase
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